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Bespoke electrophysiological and imaging equipment
Recording chambers
Compound action potential chambers
Compound action potential chambers - basic*
CAP chambers allow recording of whole nerve bundles (e.g., saphenous, sciatic, vagus, etc) from mouse and smaller nerves from rats. For bigger nerves (>400µm), a special-order chamber is available. Recording from 2 nerves simultaneously using the CAP2basic chamber is perfect for the direct comparison of two neuronal groups (such as from knock-out mice vs. wild-types, injured vs. uninjured, etc.). Not only does this chamber save time, but it also allows comparison of the groups in perfectly identical experimental conditions.
Applications
Specifications
available in either 1 (CAP1basic), 2 (CAP2basic) or 3 (CAP3basic) channels version, allowing electrophysiological recordings from either single, two or three nerves simultaneously, respectively. Nerves are gently stretched between two glass suction electrodes using a distinct application component (delivered with chamber). Electrodes are sealed with a special silicon membrane, making the electrical neuronal isolation optimal. The distance between the glass suction electrodes could be manually fixed, allowing adjustment of the chamber for nerves of varying lengths. Minimal nerve length the chamber could accommodate is 6 mm, max. – 15 mm.
* all chambers described are also available as modules, i.e. chambers can be equipped with amplifiers, heating devices, electrical stimulators, integrated sensors etc.
Compound action potential chambers - thermoregulation*
Specifications
CAP1heat (for recordings from a single nerve) or CAP2heat (for simultaneous recordings from two nerves) or CAP3heat (for triple nerve recordings - chambers could be combined with an integral heating system, allowing for complete thermoregulation of the nerves without the need of perfusing the bath with an additional external thermoregulator.
in/ex vivo/vitro electrophysiology without the need of perfusing the bath. Useful when small volumes are needed (test of expensive substances) and physiological temperature is required
Applications
* all chambers described are also available as modules, i.e. chambers can be equipped with amplifiers, heating devices, electrical stimulators, integrated sensors etc.
Compound action potential + neuropeptide release chambers*
CAP1pept (for recordings from a single nerve) - chamber could be designed with a special side port for fluid sampling, allowing determination of neuropeptide (CGRP, SP...) release alongside electrophysiological recordings. In this advanced recording chamber, a direct comparison between neuronal electrophysiological capacities and neuropeptide content/release could be made. Chamber volume is custom-made according to customer specifications within the range of 120µl – 300µl.
Specifications
Applications
in/ex vivo/vitro electrophysiology (single fiber/compound action potential from skin, muscles and visceral tissues)
Differential conduction and CGRP release in visceral versus cutaneous peripheral nerves in the mouse. De Col R, Messlinger K, Hoffmann T. J Neurosci Res. 2018 Aug;96(8):1398-1405.
Citations
* all chambers described are also available as modules, i.e. chambers can be equipped with amplifiers, heating devices, electrical stimulators, integrated sensors etc.
Changes in latency of compound action potentials (left) and simultaneous CGRP release (right) during chemical stimulation of vagus nerves
Combined compound action potential (CAP) and single fiber (sn) chambers*
* all chambers described are also available as modules, i.e. chambers can be equipped with amplifiers, heating devices, electrical stimulators, integrated sensors etc.
Applications
Combined CAP-sn chambers suit whole nerve bundles (e.g., saphenous, sciatic, vagus, etc) from mouse and smaller nerves from rats. For bigger nerves (>400µm), a special-order chamber is available.
This unique chamber pairs both CAP and sn techinques and allows a simultaneous recording from both the whole nerve, as well as its single filaments. Nerves are gently stretched between a glass electrode and a second isolated chamber section. Within this an additional suction electrode is used for the suction of fine single filaments.
Specifications
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